Make your own free website on Tripod.com
 
Name:     ID: 
 
Email: 

worksheet 10A 3rd bim

True/False
Indicate whether the statement is true or false.
 

 1. 

Growing a large number of different cells from one cell is known as cloning.
 

 2. 

Manipulating genes for practical purposes is called genetic engineering.
 

 3. 

Gene cloning is an efficient way to produce many copies of a specific DNA sequence.
 

 4. 

Scientists have used genetic engineering to produce bacteria capable of synthesizing human proteins.
 

 5. 

Gene cloning is an efficient means of producing large numbers of different genes.
 

 6. 

In bacteria, a circular DNA molecule that replicates independently of the main chromosome is called a plasmid.
 

 7. 

In the practice of genetic engineering, scientists directly manipulate genes.
 

 8. 

Plasmids are pieces of viral DNA that commonly infect human cells.
 

 9. 

DNA ligase can seal the sticky ends of a DNA fragment.
 

 10. 

Before a foreign gene is inserted into a plasmid, the plasmid is opened with a restriction enzyme.
 

 11. 

Recombinant DNA is made when a DNA fragment is put into the DNA of a vector.
 

 12. 

Restriction enzymes make a straight cut through both strands of DNA.
 

 13. 

Gel electrophoresis separates DNA fragments by their size and shape.
 

 14. 

In a Southern blot, the DNA from each bacterial colony is isolated and cut into fragments by probes.
 

 15. 

Factor V3 is a protein that promotes blood clotting.
 

 16. 

Injection of a particular vaccine can cause the body to produce antibodies that protect against the possibility of future infection by a particular pathogen.
 

 17. 

DNA fingerprinting enables genetic engineers to arrange genes in a particular order on a chromosome.
 

 18. 

DNA fingerprint analysis can be used to determine whether two individuals are related.
 

 19. 

RFLPs are pieces of DNA that are all the same length.
 

 20. 

If a crop is made glyphosate-resistant, treating it with glyphosate will seriously reduce its yield.
 

 21. 

Despite the potential environmental benefits, genetic engineers have been unable to develop crop plants that are resistant to weedkillers.
 

 22. 

Genetic engineering techniques can be used to make crops resistant to destructive insects.
 

 23. 

Genetic engineers have developed a method of infecting cows with milk-producing bacteria to increase the amount of milk produced by the cows.
 

 24. 

Dairy cattle will produce more milk when genetically engineered growth hormone is added to their food.
 

 25. 

A transgenic animal is an animal with foreign DNA in its cells.
 

Multiple Choice
Identify the choice that best completes the statement or answers the question.
 

 26. 

A strand of DNA formed by the splicing of DNA from two different species is called
a.
determinant RNA.
c.
plasmid DNA.
b.
recombinant DNA.
d.
restriction RNA.
 

 27. 

Which of the following procedures is not a usual step in a genetic engineering experiment?
a.
inducing a mutation in a source chromosome
b.
cleaving DNA with a restriction enzyme
c.
recombining pieces of DNA from different species
d.
cloning and screening target cells
 

 28. 

Genetic engineering refers to the process of
a.
creating new DNA molecules from nucleotide sequences.
b.
rearranging nucleotides in a gene of an organism so that new traits appear in the development of an embryo.
c.
moving genes from a chromosome of one organism to a chromosome of a different organism.
d.
building a new species by combining genes of different organisms.
 

 29. 

Cohen and Boyer transferred a gene from a frog chromosome into the genetic material of a
a.
different frog.
b.
different chromosome of the same frog.
c.
virus taken from the same frog.
d.
bacterial cell.
 

 30. 

The use of genetic engineering to transfer human genes into bacteria
a.
is impossible with current technology.
b.
causes the human genes to manufacture bacterial proteins.
c.
results in the formation of a new species of organism.
d.
allows the bacteria to produce human proteins.
 

 31. 

Cloning is a process by which
a.
undesirable genes may be eliminated.
b.
many identical protein fragments are produced.
c.
a virus and a bacterium may be fused into one.
d.
many identical cells may be produced.
 

 32. 

Plasmids
a.
are circular pieces of bacterial DNA.
b.
can replicate independently of the organism’s main chromosome.
c.
are often used as vectors in genetic engineering experiments.
d.
All of the above
 

 33. 

Recombinant DNA is formed by joining DNA molecules
a.
from two different species.
b.
from two chromosomes of the same organism.
c.
with RNA molecules.
d.
with proteins from a different species.
 

 34. 

plasmid : DNA segment coding for an enzyme ::
a.
DNA ligase : double-stranded DNA
b.
vector : restriction enzyme
c.
cloned cell : DNA ligase
d.
recombinant DNA : DNA from another organism
 

 35. 

Restriction enzymes are specific in their action on
a.
DNA.
c.
proteins.
b.
amino acids.
d.
chromosomes.
 

 36. 

Enzymes that cut DNA molecules at specific places
a.
have sticky ends.
b.
are restriction enzymes.
c.
work only on bacterial DNA.
d.
always break the DNA between guanine and adenine.
 

 37. 

After cloning bacteria that had been exposed to the recombinant DNA, Cohen and Boyer added tetracycline to the culture in order to
a.
kill any contaminating viruses.
b.
kill cells that did not have the recombinant DNA in their genomes.
c.
neutralize any frog genes that might remain.
d.
make the bacterial cells multiply faster.
 

 38. 

DNA fragments cut by a restriction can
a.
pair up and join with any other DNA fragments cut by the same restriction enzyme.
b.
pair only with fragments formed by a complementary restriction enzyme.
c.
combine with any other spliced chromosome.
d.
pair only with DNA from the same species.
 

 39. 

Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called
a.
probes.
c.
vaccines.
b.
plasmids.
d.
clones.
 

 40. 

A technique that uses radioactively labeled DNA to identify specific genes in a piece of DNA is called the
a.
Northern blot.
c.
Northern lights.
b.
Southern vector.
d.
Southern blot.
 

 41. 

The goal of the Human Genome Project is to
a.
create maps showing where genes are located on human chromosomes.
b.
create maps showing where chromosomes are located on human genes.
c.
treat patients with genetic diseases.
d.
identify people with genetic diseases.
 

 42. 

A medical condition that can be treated by using proteins produced through genetic engineering is
a.
diabetes.
c.
hemophilia.
b.
ovarian cancer.
d.
All of the above
 

 43. 

genetic engineering : human health ::
a.
vaccine : anticoagulant
c.
anticoagulant : hemophilia
b.
human insulin : diabetes patients
d.
diabetes : insulin
 

 44. 

factor VIII : hemophilia ::
a.
Factor VIII : diabetes
c.
blood factors : viruses
b.
growth hormone : diabetes
d.
insulin : diabetes
 

 45. 

Antibodies
a.
prevent diseases caused by vaccines.
b.
are produced by bacteria that infect animals.
c.
help destroy microbes that invade the body.
d.
cause viruses to infect bacterial cells.
 

 46. 

The risk associated with vaccines prepared by injecting killed or weakened pathogenic microbes is that
a.
a few remaining live or unweakened microbes could still cause the disease.
b.
the antibodies that result may not work.
c.
the vaccine protects only against other diseases.
d.
None of the above
 

 47. 

DNA fingerprinting has been used in criminal investigations because
a.
criminals leave DNA samples behind them when they touch an object at a crime scene.
b.
DNA analysis allows investigators to distinguish body cells of different individuals, who are unlikely to have the same DNA.
c.
bacterial DNA on the hands of criminals may provide a clue as to where that person was when the crime was committed.
d.
DNA found on murder weapons is easy to identify.
 

 48. 

A gene that codes for resistance to glyphosate has been added to the genome of certain plants. These plants will
a.
produce chemicals that kill weeds growing near them.
b.
die when exposed to glyphosate.
c.
convert glyphosate to fertilizer.
d.
survive when glyphosate is sprayed on the field.
 

 49. 

Which of the following is not an example of gene technology used in farming?
a.
the use of cow growth hormone produced by bacteria to increase milk production in cows
b.
the development of larger and faster-growing breeds of livestock
c.
the cloning of human brain cells from selected farm animals
d.
the addition of human genes to farm-animal genes to obtain milk containing human proteins
 

 50. 

Ian Wilmut’s cloning of Dolly in 1997 was considered a breakthrough in genetic engineering because
a.
scientists thought cloning was impossible.
b.
scientists thought only fetal cells could be used to produce clones.
c.
scientists had never before isolated mammary cells.
d.
sheep had never responded well to gene technology procedures.
 



 
         Start Over